Journal: eLife

Article Title: Murine alveolar macrophages rapidly accumulate intranasally administered SARS-CoV-2 Spike protein leading to neutrophil recruitment and damage

doi: 10.7554/eLife.86764

Figure Lengend Snippet: ( A ) Tissue photographs of lungs collected 2.5 hr post Spike protein (-CoV-2 Trimer), the S1 subunit of the human coronavirus (HKU1 S1), or saline instillation. Evans blue (200 µl of 5 mg/ml, PBS) was injected i.v. 1.5 hr post intranasal Spike administration. Lungs collected 1 hr after Evans blue injection. Evans blue amounts in lung and liver tissue are shown. ( B ) Confocal micrographs of lung collected at 18 hr post Spike protein. Alveolar macrophages (AMs) and neutrophils detected with Siglec-F and Ly6G antibodies, respectively. Scale bar, 500 μm. ( C ) Confocal micrographs of lung collected at 3 hr post SARS-CoV-2 Spike protein instillation show SARS-CoV-2 Spike protein (green), neutrophils (red), and AMs (cyan). ROI-1 and -2 (boxes) show contact between AMs and neutrophils and are enlarged in the right panels. Scale bars, 50 and 10 μm. ( D ) Confocal micrographs of lung collected at 3 hr post each SARS-CoV-2 Spike proteins instillation show SARS-CoV-2 Spike protein (green) and neutrophils (red). Left to right panels show saline (No Trimer), SARS-CoV-2 Spike protein (HCoV-2-Trimer), PNGase F-treated SARS-CoV-2 Spike protein (PNGase F Tx.), SARS-CoV-2 Spike protein purified from Kifunensine-treated cells (Kifunensine Tx.), and the S1 subunit of the human coronavirus HKU1 (HCoV-HKU1) Spike protein. ROIs in each upper panel (boxes) are enlarged (×3.2 magnification) in lower panels. Scale bars, 200 μm. Two-way ANOVA multiple comparisons, *p<0.05, (n=3). ROI, regions of interest. Figure 3—source data 1. Source data for .

Article Snippet: Recombinant DNA reagent , Human Coronavirus Spike glycoprotein Gene ORF cDNA clone expression plasmid (Codon Optimized) HCoV-HKU1 , SinoBiological , VG40021-UT , .

Techniques: Saline, Injection, Purification

Journal: eLife

Article Title: Murine alveolar macrophages rapidly accumulate intranasally administered SARS-CoV-2 Spike protein leading to neutrophil recruitment and damage

doi: 10.7554/eLife.86764

Figure Lengend Snippet:

Article Snippet: Recombinant DNA reagent , Human Coronavirus Spike glycoprotein Gene ORF cDNA clone expression plasmid (Codon Optimized) HCoV-HKU1 , SinoBiological , VG40021-UT , .

Techniques: Blocking Assay, Imaging, Staining, Recombinant, Plasmid Preparation, Expressing, Luciferase, Cell Culture, Transfection

Journal: Cell Reports

Article Title: Fc-mediated pan-sarbecovirus protection after alphavirus vector vaccination

doi: 10.1016/j.celrep.2023.112326

Figure Lengend Snippet: Venezuelan equine encephalitis virus replicon particle VRP3526 for high-titer vaccinations (A) Venezuelan equine encephalitis (VEE) RNA-based assembly scheme of VRP3526 particles. (B) Phylogenetic relationship of the spike protein amino acid sequences from CoVs that were used in this study, including common cold CoVs (green) and prepandemic/epidemic CoVs (blue). Of the β-CoVs, we generated spike proteins for both group 2A (HKU1) and 2B viruses. Of the group 2B viruses, we generated spike proteins for clade 1a (SARS-CoV, SHC014, WIV1), 2 (HKU3), and 1b (SARS-CoV-2, RaTG13) viruses. Tree generated from an amino acid multiple sequence alignment using maximum likelihood in Geneious Prime. (C) VRP3526 titers obtained in this study. Dashed line denotes minimum titer required for vaccination at 2 × 10 4 VRPs in a 10-μL footpad inoculation. (D) Amino acid percentage similarity (left in cell) and patristic phylogenetic distance (right in cell) of the main sarbecovirus spike proteins used in this study. (E) Immunofluorescent staining at 40× magnification for VEE non-structural proteins (top) and SARS-CoV-2 spike S2 domain (middle) in Vero E6 cells infected with VRPs expressing the spike proteins used in this study. White scale bar, 50 μm.

Article Snippet: Human CoV HKU1 S (isolate N5) , Sino Biological , 40606-V08B.

Techniques: Generated, Sequencing, Staining, Infection, Expressing

Journal: Cell Reports

Article Title: Fc-mediated pan-sarbecovirus protection after alphavirus vector vaccination

doi: 10.1016/j.celrep.2023.112326

Figure Lengend Snippet:

Article Snippet: Human CoV HKU1 S (isolate N5) , Sino Biological , 40606-V08B.

Techniques: Generated, Recombinant, Binding Assay, Variant Assay, Luciferase, Knock-Out, Sequencing, Construct, Software, Chromatography, Luminex, Enzyme-linked Immunosorbent Assay

Journal: bioRxiv

Article Title: Analysis of Serologic Cross-Reactivity Between Common Human Coronaviruses and SARS-CoV-2 Using Coronavirus Antigen Microarray

doi: 10.1101/2020.03.24.006544

Figure Lengend Snippet: Coronavirus antigens on microarray.

Article Snippet: Coronavirus , HKU1 , HKU1 , S1 , Q0ZME7 , HEK293 , Sino Biological , N-(AA13-756)-His-C , 40602-V08H.

Techniques: Microarray, Expressing, Construct